Structural Analysis and Identification of Novel Isoforms of the Circadian Clock Gene period in the Silk Moth Bombyx mori(Neurobiology)

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概要

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• The molecular basis of the circadian clock is an autoregulatory feedback loop in which the PAS domain-containing protein PERIOD periodically inhibits its own transcription. In the present study on PERIOD of the silk moth Bombyx mori, we have cloned two distinct period mRNA homologues with different PAS domain sequences either with or without the pentapeptide GTQEK. A period cDNA fragment first amplified by PCR exhibited a 15 bp-deleted nucleotide sequence in the PAS domain, compared with the database sequence. A possible arternative splicing mechanism was examined by PCR analyses, and a 15 bp-inserted clone was also amplified. The entire sequences of these period α and period β isoforms were then determined by the 3' anf 5' RACE methods. Isoform period α consists of a 3,324 bp oligonucleotide encoding 1,108 amino acid residues, whereas isoform period β comprises 3,309 bp corresponding to 1,103 amino acids. Isoforms period α and period β were found to be exactly identical except for the 15 bp deletion/insertion site. Such a pair of isoforms with a deletion/insertion sequence, namely two splice variants, has previously been reported only for the PERIOD proteins of the two honeybees, Apis mellifera and A. cerana. The occurrence of an alternative splicing mechanism in the B. mori period gene was hypothesized based on the genome structure recently clarified. Bombyx mori PERIOD α and β proteins are the isomers that reveal firstly the different PAS domain sequences.

• 社団法人日本動物学会の論文
• 2004-09-25

著者

• Sato S

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Kyushu University

• TAKEDA Yukimasa

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Kyushu University

• SATO Seiji

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Kyushu University

• SHIMOHIGASHI Yasuyuki

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Kyushu University

• SHIMOHIGASHI Miki

  Division of Biology, Faculty of Science, Fukuoka University

• KANEKI Atsushi

  Laboratory of Structure-Function Biochemistry, Kyushu University

• TOMINAGA Yoshiya

  Division of Biology, Faculty of Science, Fukuoka University

• Chuman Yoshiro

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Faculty and Graduate School

• Shirasu Naoto

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Faculty and Graduate School

• Matsuhima Ayami

  Laboratory of Structure-Function Biochemistry, Department of Chemistry, Faculty and Graduate School

• Shimohigashi Miki

  Division Of Biology Faculty Of Science Fukuoka University

• Shimohigashi Yasuyuki

  九州大学 理学研究院化学部門構造機能生化学

• Shirasu N

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Shirasu Naoto

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Shirasu Naoto

  Dep. Of Electronics Graduate School Of Information Sci. And Electrical Engineering Kyushu Univ.

• Mohri Shirou

  Department Of Laboratory Animal Science Faculty Of Medical Sciences Kyusyu University

• Kaneki Atsushi

  Faculty And Graduate School Of Sciences Kyushu University

• Sumiyoshi Miho

  九州大学 理学研究院構造機能生化学研究室

• Yoshiya Tominaga

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Shimohigashi Yasuyuki

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kyushu University

• Tominaga Y

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Chuman Yoshiro

  Division Of Chemistry Graduate School Of Science Hokkaido University

• Chuman Yoshiro

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Takeda Yukimasa

  Laboratory Of Structure-function Biochemistry Department Of Chemistry The Research-education Centre

• Shimohigashi Yasuyuki

  Laboratory Of Structure-function Biochemistry Department Of Molecular Chemistry Graduate School Of S

• Tominaga Yoshiya

  Division Of Biology Faculty Of Science Fukuoka University

• Sato S

  National Salmon Resources Center Fisheries Research Agency

• Sato Seiji

  Laboratory Of Structure-function Biochemistry Department Of Chemistry Faculty And Graduate School Of

• Chuman Yoshiro

  Laboratory Of Biological Chemistry Department Of Chemistry Faculty Of Science Hokkaido University

• Shirasu Naoto

  Fukuoka Univ. Fukuoka Jpn

• Shimohigashi Miki

  Division Of Biology Department Of Earth System Of Science Fukuoka University

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