コマツナ発芽試験のための堆肥からの生育阻害物質抽出法

概要

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We investigated method to efficiently extract phenolic acid and volatile fatty acid as plant growth inhibitors in order to adjust pH and remove the salts from the compost for use in Komatsuna germination test. The recovery rate of the inhibitor was compared in various conditions as a kind of organic solvent, extraction period and temperature. Preferable condition and procedure was summarized as follows. 1. Hot water extract was prepared from dried and ground compost suspended in distilled water at 60℃ for 60 minutes. 2. The same volume of compost extract and organic solvent composed of equal volumes of diethyl ether and acetic ethyl was mixed and shaken for 10 minutes. 3. After a few minutes, the supernatant layer was isolated. 4. A small volume of 0.1N-NaOH was added to the supernatant liquid and dried in water bath at 60℃. 5. Dried supernatant was dissolved in a fixed volume of hot water containing a small amount of phosphate buffer solution.
2007-04-15