透明浮遊細胞高速トラッキングのための光学的可視化技術

概要

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This paper describes an optical visualization method for a transparent object that is capable of realizing high speed tracking of a motile cell without the need to use fluorescent labeling. This method visualizes a non-labeled cell as a periodic light intensity distribution by deriving the diffracted light of a single frequency from the sample. Using this periodic pattern, a high response photodiode can be employed to measure the velocity of a transparent cell. We propose an optical spatial-filtering method to select the spatial frequency component, which introduces two pinholes in the optical Fourier-transform plane. The positions of these pinholes are the two focusing points of the ±1^<st>-order diffracted light for the selected spatial frequency. By blocking the 0^<th>-order diffracted light, which includes the regular reflection component from the surface of the cover glass that is the main source of stray light, a laser of high coherence and high power can be used as the light source. We could clearly observe three kinds of transparent cell (lymph cell: JURKAT, white blood cell: K562, breast cancer cell: YMB-1-E) as periodic light intensity distributions. In addition, we confirmed that this pattern moves in accordance with the translational movement of the cell.
社団法人精密工学会の論文
2007-04-05