A Taxon-specific Oligonucleotide Primer Set for PCR-based Detection of Soil Ciliate
スポンサーリンク
概要
- 論文の詳細を見る
A PCR primer set was designed to amplify the 18S rRNA gene from the phylum Ciliophora (ciliates). The pair amplified DNA from 5 organisms representing the major taxonomic groups of ciliates but not from 13 nonciliate organisms. To test the ability of the primers to amplify ciliate rRNA genes from environmental samples, a clone library was constructed from an agricultural soil sample and analyzed. The rRNA gene sequences of all clones were affiliated with the Ciliophora. This newly developed PCR primer set is able to amplify ciliate rRNA genes from soil, and may be useful for the detection and identification of ciliates.
- 日本微生物生態学会の論文
著者
-
笠原 康裕
北大低温研
-
Kasahara Yasuhiro
College Of Agriculture Ibaraki University : Institute Of Low Temperature Science Hokkaido University
-
Kasahara Yasuhiro
Inst. Of Low Temp. Sci. Hokkaido Univ.
-
Sato Yoshinori
College of Agric., Ibaraki Univ.
-
Shimano Satoshi
Environment Education Center Miyagi University Of Education
-
Shimano Satoshi
National Agricultural Research Center For Tohoku Region:(present Office)environmental Education Cent
-
Shimano Satoshi
Department Of Obstetrics And Gynecology Otaru Kyokai Hospital
-
MIYOSHI Norikazu
Department of Chemistry, Faculty of Integrated Arts and Sciences, The University of Tokushima
-
PUITIKA TUNJUNG
College of Agriculture, Ibaraki University
-
Miyoshi Norikazu
Department Of Upland Farming National Agricultural Research Center For Tohoku Region
-
Miyoshi Norikazu
Department Of Chemistry Faculty Of Integrated Arts And Sciences University Of Tokushima
-
Puitika Tunjung
College Of Agriculture Ibaraki University
-
Miyoshi Norikazu
Department Of Chemistry Faculty Of Integrated Arts And Sciences The University Of Tokushima
-
笠原 康裕
北海道大・低温研
-
Shimano Satoshi
Environmental Education Center, Miyagi University of Education
関連論文
- C-127 枯草菌ゲノムDNA分泌に関与するタンパク質群(バイオフィルム、生理・増殖,ポスター発表)
- ノニルフェノール類化学物質による環境汚染と分解微生物 : 土壌における実態の解明に向けて
- B-28 Sphingomonas sp.Y.T株の低濃度ノニルフェノールの分解性(バイオレメディエーション,B会場,口頭発表)
- Detection of Effects of a High Trophic Level Predator, Sorex unguiculatus (Soricidae, Mammalia), on a Soil Microbial Community in a Cool Temperate Forest in Hokkaido, Using the ARISA Method
- 04-066 土壌微生物群集に対する高次捕食者トガリネズミの効果(予報)(土壌生態系,研究発表)
- PB-71 家畜スラリーを投与した畑土壌における繊毛虫相の解析 : 土壌からの繊毛虫DNA抽出法の検討(農耕地生態系,ポスターセッションB,(1)ポスター発表会,研究発表会)
- PA-66 大腸菌の生きているが培養不能(VBNC)状態のプロテオーム解析(遺伝子解析,ポスターセッションA,ポスター発表)
- PA-57 枯草菌ペリクルの形成因子の同定(界面/バイオフィルム,ポスターセッションA,ポスター発表)
- PA-56 枯草菌のペリクル形成過程の顕微鏡学的およびプロテオーム解析(界面/バイオフィルム,ポスターセッションA,ポスター発表)
- P-035 枯草菌ペリクル構造体の形成過程と発現蛋白質プロファイル(界面・バイオフィルム,ポスター発表)
- P-030 細胞膜構成脂肪酸による枯草菌の溶菌誘導(生理・増殖,ポスター発表)
- ポストゲノム微生物研究における展開 : 枯草菌のプロテオミクス(I 土壌微生物学におけるポストゲノム研究の現状と将来, 2004年度シンポジウムならびに受賞記念講演講演要旨集)
- C-58 ノニルフェノール(NP)分解性Sphingomonas sp. YTと共存するNP非分解性細菌の分類学的研究(分類・系統解析,第2回ポスター発表)
- C-27 枯草菌形態変化細胞の発現タンパク質解析(その2) : 形態変化機構と誘導因子(環境衛生、食品衛生、生理・増殖、界面・バイオフィルム,第1回ポスター発表)
- C-26 枯草菌形態変化細胞の発現タンパク質解析(その1) : 形態変化関連遺伝子の探索(環境衛生、食品衛生、生理・増殖、界面・バイオフィルム,第1回ポスター発表)
- C-38 広瀬川鉱物粒子の表面・内部に存在する細菌の多様性(群集構造解析,C会場,ポスター発表)
- C-22 界面での枯草菌の増殖様式(生理・増殖,C会場,ポスター発表)
- C-17 Bacillus subtilis遺伝子変異株の細胞表面特性の解析(界面・バイオフィルム,C会場,ポスター発表)
- C-206 広瀬川河原に存在するSMCP(2) : 系統解析による内部細菌の多様性(土壌生態系-3,ポスター発表)
- 4-32 草地土壌中の低栄養微生物(4.土壌生物)
- Cytofluorometric quantitation of SH and SS groups in the mouse hepatocyte nuclei
- Active Oxygens and Cancer Cachexia
- Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy
- 1-04 Effect of phylogenetic diversity using multiple displacement amplification for bacterial community structure(Community structure 1,Oral presentation)
- Reinforcement Therapy Using Nitric Oxide Synthase Inhibitors Against Endotoxin Shock in Dogs
- P-9 Studies on histopathological findings, coagulofibrinolytic system, Cytokine levels and tissue NO generation produced by bleeding invasion of the rat : verification on occurrence of DIC
- Cell proliferation and cytodifferentiation of rat thyroid tumors
- Simultaneous Cytofluorometry of SH and SS groups in nuclear protein and DNA of mice human hepatocytes(IV)
- Bioimaging of protein secondary structure from Fourier transform infrared spectroscopy in experimental tumor tissue
- Macrophage Photodynamic Treatment Reaction Alters Nitric Oxide Production and Tumoricidal Activity
- P-44 Expression of bcl-2 and P53 in human colorectal adenomas and carcinomas : a reciprocal evidence in tumorigenesis
- Histopathological study of cachectic rat : Multi-Organ Damage( MOD ) induced by cancer cachexia
- Development of multipurpose automethod cell staining system.
- AO Fragile sites on the chromosome of normal human lymphocyte and CML culter cell
- A New Nuclear RNA(nRNA)- A Gene Suppressor in the Process of Irreversible Cytodifferentiation
- Detection of Effects of a High Trophic Level Predator, Sorex unguiculatus (Soricidae, Mammalia), on a Soil Microbial Community in a Cool Temperate Forest in Hokkaido, Using the ARISA Method
- PB-50 Carbonyl sulfide (COS)-degrading activity and phylogenetic identity of the bacterial isolates from the MPN for counting COS-degrading microbes in soils(Matter cycling,Poster presentation B)
- PA-27 Microbial nitrous oxide emission from agricultural soils under different nitrogen application(Agricultural ecosystem,Poster presentation A)
- 1-24 Effect of copper nonylphenol sulfonate on soil microbial communities(Community structure 2,Oral presentation)
- 1-13 Denitrification activities of fungal isolates from agricultural soils.(Agricultural ecosystem,Oral presentaion)
- Herniation of Cervical Intervertebral Disc : Immunohistochemical Examination and Measurement of Nitric Oxide Production
- Analysis of the prevalence of bacterial vaginosis and Chlamydia trachomatis infection in 6083 pregnant women at a hospital in Otaru, Japan
- Possible association between screening BV at the prenatal visit and reduced cervical cerclage : Multi-center questionnaire in Hokkaido, Japan
- Effects of Light-dose and Wavelengths of Photodynamic Therapy with Photosensitizer on Induction of Apoptotic Cell Death
- P-35 An Interaction of the Apoptosis and the Kinds of Active Oxygen Species Produced by Photosensitization with Laser Light and Photosensitizer
- Multicolor staining of root systems in pot culture(Plant Nutrition)
- TREATMENT CONDITIONS OF THE SOIL SAMPLES IN THE ESTIMATION OF THE BIOMASS OF SOIL CILIATES USING THE MPN METHOD(Behavior Biology and Ecology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Water Accelerated Allylation of Aldehydes by Using Water Tolerant Grignard-Type Allylating Agents from Allylmagnesium Chloride and Various Metallic Salts
- A Convenient One Pot Allylation of Aldehydes with Allylic Halides Prepared in Situ from Allylic Alcohols in the Presence of Metallic Bismuth
- The Cross-Coupling Reaction of Aldehydes with α-Diketones by the Use of Bismuth Trichloride and Metallic Zinc
- Solvent-free Imino-Aldol Three-component Couplings on a Conveniently-prepared and Reusable Phosphoric Acid-Silica Gel Support
- Solvent-Free One-Pot Reduction of Imines Generated in situ from Aldehydes and Aniline by Tributyltin Hydride on Silica Gel
- Bismuth Trichloride Catalyzed Efficient Reductive Etherification of Carbonyl Compounds with Alcohols : A Novel Method for Preparation of Symmetrical and Unsymmetrical Ethers
- A Grignard-Type Addition of Allyl Unit to Carbonyl Compounds Containing a Carboxyl Group by Using BiCl_3-Zn(0)-Allyl Bromide
- Earthworm Fauna of Daikokujima Island, North Japan, with a Redescription of Amynthas yunoshimensis (Hatai, 1930) : Annelida, Clitellata, Megascolecidae
- The Chemistry of Alkylstrontium Halide Analogues : Barbier-type Alkylation of Imines with Alkyl Halides
- The Barbier-Type Allkylation of Aldehydes with Alkyl Halides in the Presence of Metallic Strontium
- A Case of Simultaneous Bilateral Putaminal Hemorrhage
- Differences of IgG and IgM Antibodies to Phospholipids and APTT among Non-Pregnant and Pregnant Women
- A New Synthetic Method for Para Alkylation of Benzoic Acids Using Metallic Strontium and Alkyl Iodide
- Ophiocordyceps pulvinata sp. nov., a pathogen of ants with a reduced stroma
- Linkage between Light Microscopic Observations and Molecular Analysis by Single-Cell PCR for Ciliates
- A Taxon-specific Oligonucleotide Primer Set for PCR-based Detection of Soil Ciliate
- PB-05 Analysis of soil ciliates community using 18S rDNA(MICROBL COMMUNITY ANALYSIS,Session B,(1) Poster presentations)
- P-059 Community Analysis of Ciliates in Soil Based on 18S rDNA
- Occurrence of Camisia solhoeyi (Oribatida:Camisiidae) in Japan
- Reductive Self-coupling Reaction of Imines and Aldehydes Induced by Strontium Metal
- A New Synthetic Method for Methyl Ketones from Carboxylic Acids Using Metallic Strontium and Methyl Iodide
- Oribatid mites (Acari : Oribatida) as an intermediate host of Anoplocephalid cestodes in Japan
- Two Cases of Intestinal Ischemia Associated with Hepatic Portal Venous Gas
- Improvement of root staining method for field applications(Plant nutrition)
- IS THE JAPANESE ORIBATID MITE EUPHTHIRACARUS FOVEOLATUS AOKI, 1980 (ACARI: EUPHTHIRACARIDAE) A JUNIOR SYNONYM OF E. CRIBRARIUS (BERLESE, 1904)?(Taxonomy and Systematics,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society
- Sphingomonas amiensisとSphingomonas cloacaeによるノニルフェノール分解特性の解析(2003年度大会一般講演要旨)
- 難培養性細菌のゲノム解析を目指して : 1細胞微量ゲノム増幅法の検討(2006年度大会一般講演要旨)
- カバークロップが土壌微生物相に及ぼす影響について(2005年度大会一般講演要旨)
- A Case of Subclavian Artery Injury Due to Blunt Trauma Treated Successfully by Endovascular Repair
- The Convenient and Useful Esterification of Bulky Tertiary Alcohols Using Strontium Compounds
- Discrepancy between conventional morphological systematics and nuclear phylogeny of tintinnids (Ciliophora : Choreotrichia)