Analysis of Bud Sport Cultivars of Peach (Prunus persica (L.) Batsch) by Simple Sequence Repeats (SSR) and Restriction Landmark Genomic Scanning (RLGS)
スポンサーリンク
概要
- 論文の詳細を見る
To detect genetic mutations in bud sport cultivars of peach, RLGS (restriction landmark genomic scanning) analysis was performed on 7 putative bud sport mutant cultivars after SSR (simple sequence repeats) analysis for identifying false-mutants. Three cultivars, 'Yawata Hakuho', 'Odoroki', and 'Kanoiwa Hakuto', postulated to be bud sport mutants, were confirmed as false-mutant cultivars. This suggests that other false-mutant strains of peach are also likely to be registered with the Plant Variety Authority as bud mutant cultivars. A stable RLGS profile of peach, obtained using Not I as a landmark enzyme, showed about 400 spots in a single experiment. Comparing the profiles between mutant and original cultivars, polymorphic spots were discovered in 2 mutatnt cultivars, 'Gyosei' and 'Nagasawa Hakuho', but were not detected by SSR analysis. RLGS was thus considered to be an effective method to detect very small genomic variations in mutant strains.
- 園芸学会の論文
著者
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Sato Y
Department Of Clinical Physiology Tokyo Metropolitan Institute Of Gerontology
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Sato Yo-ichiro
Res. Inst. Of Humanity And Nature
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Sato Y
Yamanashi Medical Univ. Yamanashi Jpn
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Matsuta Nagao
National Institute of Fruit Tree Science
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Iketani Hiroyuki
National Agricultural and Bio-oriented Research Organization
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Sato Yoshihiko
National Institute of Fruit Tree Science
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Mase Nobuko
National Institute of Fruit Tree Science, National Agriculture and Food Research Organization
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Mase Nobuko
National Institute Of Fruit Tree Science (nifts)
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Mase Nobuko
National Institute Of Fruit Tree Science
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Iketani Hiroyuki
National Institute Of Fruit Tree Science National Agriculture And Food Research Organization
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Iketani Hiroyuki
National Institute Of Fruit Tree Science (nifts)
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Sato Y
National Institute Of Fruit Tree Science
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Sato Yoshihiko
National Institute Of Fruit Tree Science (nifts)
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Mase Nobuko
National Inst. Of Fruit Tree Sci. National Agriculture And Food Res. Organization
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