サイトメガロウイルス感染症の迅速DNA診断法の開発

概要

論文の詳細を見る
The conventional diagnostic procedures for CMV infections have been both isolation of viruses and titeration of serum antibodies. These methods need several days for the final diagnosis, causing a delay in the diagnosis. Therefore, a rapid diagnostic procedure has been highly required. In this study, we conducted polymerase chain reaction (PCR) using three sets of primers corresponding IE-, LA- and V-regions of the CMV genome. Using the former two sets of primers, many bands were amplified from a DNA sample isolated from the peripheral blood leukocytes of a patient with CMV infection. However, using the latter set of primers (V-region), a single band of 305bp was amplified. Using these V-region primers, no hybridization was needed to detect the specific band. No PCR amplification products was detected by using the V-region primers from DNAs isolated from normal subjects. We also tried to use urine sedimentation samples without extraction of DNA. Sedimented cells were resuspended in water, heated by a microwave oven and used for PCR templates. A 305bp band was also detected. Subsequently, the entire procedure of our method to detect the CMV DNA requires only 4 hours. Therefore, this PCR method is reliable and useful in making rapid diagnosis of CMV infection.
武庫川女子大学の論文