Removal of Chlorophenols by Fungal Laccase in the Presence of Aromatic Alcohols

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The effect of aromatic alcohols, coniferyl, sinapyl, vanillyl and iso-vanillyl alcohols, on the removal of chlorinated phenols from water environment by fungal laccases from Cerrena unicolor and Rhizoctonia praticola was studied. In optimal conditions all tested alcohols removed about 30 to 60% of chlorophenols from the supernatant, compared to that of laccase alone. R. praticola at pH 7.0 significantly removed more chlorophenols from supernatant than in the case of C. unicolor at pH 5.5. The laccase reaction is independent on the enzyme source and favours higher pH condition. Tested chlorophenols were partly coupled with aromatic alcohols and sedimented. It almost linearly depended on amounts of chlorine atoms in chlorophenols; more chlorine atoms resulted in higher yield of coupling reaction.
九州大学の論文