Regulation of FoxO1 Transcription Factor by Nitric Oxide and Cyclic GMP in Cultured Rat Granulosa Cells(Reproductive Biology)

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概要

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• FoxO1 is a transcription factor implicated in a multitude of physiological processes including cell cycle progression, apoptosis and insulin signaling. Recent findings indicate that FoxO1 is a key regulator during the proliferation and maturation of granulosa cells. Over the past several years, it has become evident that nitric oxide (NO) and cGMP modulate ovarian function. There has been no information, however, about whether NO-cGMP affects FoxO1 expression or about the relationship between NO-cGMP and FoxO1. In the present study, we used immunoblot analysis to determine whether NO and cGMP affect FoxO1 expression in cultured granulosa cells. Our results clearly showed that FSH suppressed FoxO1 expression in a time-dependent manner, and that NO-cGMP stimulated FoxO1 expression in cultured granulosa cells. In addition, this stimulatory effects of NO and cGMP can be blocked by FSH in cultured granulosa cells. These findings demonstrate that NO and cGMP influence FoxO1 expression possibly through antagonizing the action of FSH in cultured granulosa cells. Results of both immunoblot analysis and immunohistochemistry also show that estradiol implantation do not affect the expression of FoxO1 in rat granulosa cells as gonadotrophins do, indicating that mechanism of estradiol on granulosa cells is different from gonadotrophins. Together, our experiments suggest that expression of FoxO1 in rat granulosa cells can be regulated by gonadotrophins and the NO/cGMP signaling pathway.

• 社団法人日本動物学会の論文
• 2005-12-25

著者

• Hutz Reinhold

  Department Of Biological Sciences University Of Wisconsin-milwaukee

• Wang Zhengchao

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Shi Fangxiong

  Lab. Of Animal Reproduction Coll. Of Animal Sci. And Technol. Nanjing Agricultural Univ.

• Shi Fangxiong

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Wang Zhengchao

  中華人民共和国

• Hutz Reinhold

  Department Of Biological Sciences Niehs Marine And Freshwater Biomedical Sciences Center University

• Li Xuebin

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• LIU Gentao

  Maxine Dunitz Neurosurgical Institute, Cedars-Sinai Medical Center, University of California

• Jiang Yongqing

  Institute of Animal Sciences and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences

• Liu Wenbin

  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural Un

• Xie Zhuang

  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural Un

• Liu Gentao

  Maxine Dunitz Neurosurgical Institute Cedars-sinai Medical Center University Of California

• Liu Wenbin

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Jiang Yongqing

  Institute Of Animal Sciences And Veterinary Medicine Zhejiang Academy Of Agricultural Sciences

• Liu Gentao

  Maxine Dunitz Neurosurgical Institute Cedars-sinai Medical Center

• Liu Wenbin

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Xie Zhuang

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Shi Fangxiong

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Li Xuebin

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Wang Zhengchao

  Laboratory Of Animal Reproduction College Of Animal Science And Technology Nanjing Agricultural Univ

• Xie Zhuang

  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University

• Liu Gentao

  Maxine Dunitz Neurosurgical Institute, Cedars-Sinai Medical Center:Division of Hematology Oncology, Cedars-Sinai Medical Center David Geffen School of Medicine at UCLA

• Li Xuebin

  Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University

関連論文

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• TCDD Increases Inhibin A Production by Human Luteinized Granulosa Cells In Vitro
• Reproductive Endocrine Disruption by Environmental Xenobiotics that Modulate the Estrogen-Signaling Pathway, Particularly Tetrachlorodibenzo-p-dioxin (TCDD)
• Regulation of the Aromatic Hydrocarbon Receptor (AHR) by In-Utero and Lactational Exposure to 2, 3, 7, 8-Tetrachlorodibenzo-p-Dioxin (TCDD)
• Cyclic Changes in Serum Follicle-Stimulating Hormone, Luteinizing Hormone and Inhibin During the Guinea Pig Estrous Cycle
• Regulation of the Aromatic Hydrocarbon Receptor (AHR) by In-Utero and Lactational Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)
• Cellular Localization of Inhibin α-subunit, PKB/Akt and FoxO3a Proteins in the Ovaries of Minipigs
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• Localization of Ovarian Inhibin/Activin Subunits in Follicular Dominance during the Estrous Cycle of Guinea Pigs : Reproductive Biology
• Development of Embryos in Superovulated Guinea Pigs following Active Immunization against the Inhibin α-Subunit
• Regulation of FoxO1 Transcription Factor by Nitric Oxide and Cyclic GMP in Cultured Rat Granulosa Cells(Reproductive Biology)
• Short-Term Hormone Release from Adult Female Rat Hypothalamic and Pituitary Explants is not Altered by 2,3,7,8-Tetrachlorodibenzo-p-dioxin
• Effects of Follicle-Stimulating Hormone Administration on Oestradiol-Induced Cystic Ovaries in Guinea Pigs
• Oestradiol Exerts Disparate Effects on Ultrastructure and Steroidogenesis of Ovarian Theca from Preovulatory Hamster Follicles(Endocrinology)
• Contribution of hypoxia-inducible factor-1α to transcriptional regulation of vascular endothelial growth factor in bovine developing luteal cells
• Retraction: Regulation of FoxO1 Transcription Factor by Nitric Oxide and Cyclic GMP in Cultured Rat Granulosa Cells.

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