^1H NMRの代謝実験への応用 : リンゴ食が尿成分に与える影響の検討

概要

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A study was made to uncover the dietary influence of apple diet on the chemical composition of human urinary excretion by using ^1H NMR spectroscopy. Three subjects participated in a 6 day metabolic study, where the first 3 days was a normal diet and the latter three days was an apple diet. The amount excreted of each metabolite in the urinary samples of the three subjects collected every half day was not always proportional to the creatinine content, except for citrate which showed a good linear relationship between them with R^2=0.90. The results suggested that no dietary influence was expected for endogeneous citrate in contrast to other metabolites. The amount excreted of each metabolite in every half day sample varied regulary depending on day or night: the amount during nighttime tended to be less than that during daytime, including creatinine for two subjects, while the reverse was observed for one subject, although the tendency was less regular. The amount of excreted metabolites increased appreciably with the apple diet for one subject, and was almost unchanged for the other two subjects. Principal component analysis of all the urinary samples gave a grouping corresponding to each subject on the principal component map. Urinary samples from each subject also had a tendency to gather in two groups depending on the period of apple diet or normal diet, and on day or night.