Triton-Gel Electrophoresis of Histones Containing Oxidized Methionine and Cysteine Residues

スポンサーリンク

概要

• 論文の詳細を見る

• Electrophoresis in polyacrylamide gels in the presence of 6mM Triton X-100 in 0.9M acetic acid and 6.25M urea gave a high resolution of separation of the histones in which methionines and cysteines, respectively, are oxidized to the sulfoxides or sulfones (H2A, H2B, H3, H4) and to form an intramolecular disulfide bond (H3). The methionine-oxidized histones migrated faster than untreated histones. The electrophoretic mobility of histone H3 also increased by oxidation of the two cysteines to a cystine. The increase of the mobilities might be explained in terms of the decrease of the helical contents of the histones by the oxidation of methionine and/or cysteine residues.

• 群馬大学の論文
• 1984-03-30

著者

• 浜名 康栄

  College Of Medical Care And Technology And The Institute Of Endocrinology Gunma University

関連論文

• 生命倫理と教育 : 研究活動報告(1983.1〜1983.12)
• Triton-Gel Electrophoresis of Histones Containing Oxidized Methionine and Cysteine Residues
• Effect of the Degree of Cross-Linkage of Polyacrylamide Gel on the Electrophoretic Mobility of Histones
• 生命化学研究と化学教育の一体化 : 研究活動報告
• Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants
• 生命科学と医療技術 : 研究活動報告No.1(1978.4〜1980.12)(研究活動)
• Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines
• ニジマス肝臓におけるエストロゲン結合部位
• ブタ甲状腺クロマチンのヌクレオソームへのHMG2蛋白質の分布

スポンサーリンク