Effect of the Degree of Cross-Linkage of Polyacrylamide Gel on the Electrophoretic Mobility of Histones

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概要

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• The electrophoretic mobility in polyacrylamide gel of calf thymus histones was examined as a function of the degree of cross-linkage (C,%) of the gel in five different buffer systems. With increasing the degree of cross-linkage, the mobility once decreased and then increased, irrespective of total gel concentrations (T,%) used (5-15%). The degree of cross-linkage of the gel where the mobilities of the histones became minimum (C_<min>), 7-15%C, was larger than that where the mobilities of other high molecular weight proteins become minimum, 5-7%C. The retardation coefficients (K_R) of the histones obtained from changes of the total gel concentration attained their maximum values in gels which have C_<min>. In gels of C_<min> an approximately linear relationship was obtained between the K_R and the molecular weight of the histones.

• 群馬大学の論文
• 1983-03-30

著者

• 浜名 康栄

  College Of Medical Care And Technology And The Institute Of Endocrinology Gunma University

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