Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines

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概要

• 論文の詳細を見る

• Polyacrylamide gel electrophoresis or chromatography of histories (H1, H5, H2A, H2B, H3 and H4) and protamines (salmine and clupeine) in 0.9M acetic acid or 0.01M HC1, respectively, was carried out at various temperatures. A retardation of either electrophoretic migration or chromatographic elution of the proteins was observed at lower temperatures. H1 and H5 histones exhibited the least extent of retardation. The retardation with lowering temperature was also observed in the presence of 0.02-0.05M NaCl but not in the presence of 5-6.25M urea. The extent of retardation in polyacrylamide gel chromatography with lowering temperature increased with proteins of higher arginine contents. The fact was explained as that ionic interactions between guanido groups of arginine residues in the proteins and carboxyl groups of polyacrylamide are strengthened at lower gel temperatures.

• 群馬大学の論文
• 1981-03-10

著者

• 浜名 康栄

  College Of Medical Care And Technology And The Institute Of Endocrinology Gunma University

関連論文

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• 生命科学と医療技術 : 研究活動報告No.1(1978.4〜1980.12)(研究活動)
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