Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants

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概要

• 論文の詳細を見る

• Histones and protamines behaved as a monomeric form in 0.9M acetic acid. When gel electrophoresis was carried out in 0.9M acetic acid, the mobilities of calf H2A, H2B, H3 and H4 histones were decreased proportionaly to the helix content of the histones but the mobilities of calf H1 histone and fish protamines were not decreased by the addition of 6mM nonionic surfactant into the gel. Degree of the decreasing on the mobilities of four histones was also proportional to the molecular weight of the added nonionic surfactant. Structure of hydrophobic group of the surfactants was more affective than polyoxyethylene chain length of the surfactants. H2B, H3 and H4 histones aggregated in 0.05-0.1M sodium phosphate buffer, pH 7.0, while H1 and H2A histones behaved as a monomeric form. Nonionic surfactants bound to H2A histone but did not to H1 histone and to the aggregates of other histones in the buffer.

• 群馬大学の論文
• 1982-03-10

著者

• 浜名 康栄

  College Of Medical Care And Technology And The Institute Of Endocrinology Gunma University

関連論文

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• 生命化学研究と化学教育の一体化 : 研究活動報告
• Gel Electrophoresis, Gel Chromatography and Sedimentation Analysis of Histones and Protamines in the Presence of Nonionic Surfactants
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• Effect of Temperature on Polyacrylamide Gel Electrophoresis and Chromatography of Histones and Protamines
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