ウサギ諸臓器・組織における蛋白質燐酸化酵素Cアイソザイムの免疫組織化学的研究

概要

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Immunoreactive protein kinase C (PKC) isozymes in normal rabbit tissues were immunohistochemically localized by the peroxidase anti-peroxidase complex method using monoclonal antibodies against rabbit brain PKC isozymes I, II, and III (PKC-I, II, and III). Immunoreactive PKC-I was present almost exclusively in the brain and PKC-II immunoreactivity was present predominantly in the lymphatic and reticuloendothelial systems. Definite staining for PKC-II isozyme was also found in chondrocytes of the cartilage and endocardial cells of the heart. Immunoreactive PKC-III-like molecules were distributed widely in many organs but more densely in particular portions or specific cells of the epithelial system as shown by intense staining in the pancreatic duct and macula densa of the renal tubuli. Differential distribution of the PKC isozymes in distinct cells or specific structures within each organ or tissue, such as PKC-II expression on the hepatic sinusoidal lining cells and PKC-III expression on the intrahepatic bile duct epithelium of the liver, was also indicated. These results provided new evidence for distinct patterns of tissue distribution and cellular or subcellular location of the isozymes of PKC in various tissues and suggested specific functions of each of the isoenzymes in the tissues and organs as well as the usefulness of immunohistochemical detection of PKC in a pathological study.
群馬大学の論文
1994-03-31