テトラヒメナのヒストンのメチル化、アセチル化、およびリン酸化

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Labeled ε-N-acetyl-Lys, ε-N-monomethyl-Lys, ε-N-dimethyl-Lys, 3-methyl-His, ω-N-monomethyl-Arg, O-phospho-Ser and O-phospho-Thr were detected in the hydrolysates of the isolated whole histones from Tetrahymena pyriformis GL cultured in the presence of ^3H-acetic acid, L-methionine (methyl-^3H) and ^<32>P-H_3PO_4. When the isolated Tetrahymena nuclei were incubated with S-adenosyl-Met (methyl-^3H), ^3H-acetyl-CoA and γ-^<32>P-ATP, labeled ε-N-monomethyl-Lys, ε-N-acetyl-Lys and O-phospho-Ser were also found in the histone hydrolysates. The methylation was found in H3, H4 and H1 histones; acetylation in H2A, H2B, H3 and H4; phosphorylation in H2A, H4 and H1. The phosphorylation activity found in the nuclei isolated from the expornentially growing cells was higher than that found in the nuclei from the cells at stationary phase or starvation. The phosphorylation of H1 in the isolated nuclei was stimulated by the presence of 1×10^<-5> M of cyclic AMP or cyclic GMP while the modification of other histones was not stimulated by the cyclic mononucleotides.
群馬大学の論文
1986-03-30

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