Genetic diversity and conservation of the threatened freshwater Clown Knifefish Notopterus chitala through artificial propagation in the Mekong Delta

概要

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In order to evaluate and to recover the gene pool of the threatened Clown Knifefish, listed in the red data book of Viet Nam, we applied an inducing hormone, LRH-A3+Dom at three different decisive rates of 70mg, 100mg, and 150mg LRH-A3/kg for female fish at half of the decisive rates of those applied for male fish. In parallel, we also caught randomly ten Clown Knifefishes from four ponds and analyzed them using the SDS-PAGE electrophoretic protein method to evaluate their genetic diversity. Results showed that most of the electrophoretic proteins of the samples were similar to each other, suggesting that the gene pool of this species is very narrow and may decline seriously in the near future. Injecting the inducing hormone hormone at a rate of 150mg LRH-A3/kg led to high rates (about 95%) of fertilization and hatching. Fortunately, we detected differently the genital papilla of mature males and females during the spawning season. Given the successful results of artificial propagation and the economic significance of this fish as food, this species may perhaps be encouraged to increase and so may be removed from the red data book in the near future.
野生生物保護学会の論文