Seed storage protein diversity of three rice species in the Mekong Delta

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Today, wild rice species, Oryza rufipogon, O. officinalis, and floating rice, O. sativa, have become scarce rapidly in the Mekong Delta. In order to evaluate and to conserve genetic resources of two wild rice species distributed in the Mekong Delta, we compared their morphological features and examined protein variations by using SDS-PAGE analysis method with cultivated rice, weedy rice (O. sativa and two recombinant inbred lines RIL 93-1 and RIL 123-1), which were crossed between two species, O. officinalis and O. sativa. Results showed that the morphological materials including O. officinalis specimens were different in two distinctive characteristics from those reported before, such as thick rhizome, green grain color. O. officinalis has more heavier molecular weight in some proteins (glutelin, prolamin) than the others. In particular, protein profiles of O. officinalis populations showed a complete lack of prolamin 16kDa, less 13kDa and low waxy content compared with the other species. Two RIL lines detected at F_6 generation, have high waxy protein content, indicating that waxy content is still a complex characteristic but it may be applicable for improvement of rice amylose content. Today, wild rice species, especially O. officinalis, have become scarce in the Mekong Delta. Urgent conservation of these wild species and intensive survey of diversity are necessary.
野生生物保護学会の論文