An approach to the conservation and exploitation of useful genetic diversity of the Knifefish Notopterus notopterus Pallas in the Mekong Delta of Viet Nam

概要

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In order to evaluate the genetic diversity and to recover the gene pool of the Knifefish Notopterus notopterus in the Mekong River delta, we used protein electrophoresis SDS-PAGE. Following the detection of different genital papillae of mature males and females during the spawning season, we initially applied artificial propagating medicine by using hormone LRH-A3+Dom type to deduce whether fertilization and hatching occurred or not. Results showed that most of the protein profile samples were similar to each other, indicating that the genetic diversity of this species is already narrow and likely to decline further in the near future. The inducing hormone LRH-A3+Dom was applied at three different decisive rates of 70μg, 100μg, and 150μg LRH-A3/kg for 10 female fish at half of the decisive rates used for three male fish. The results revealed that inducing hormone used at a rate of 150mg LRH-A3/kg gave the highest percentages of fertilization and hatching (100%). Given our initial successful results of artificial propagation, and the species' economically important food value, this species could become an important fish species, commercially speaking, in the near future.
野生生物保護学会の論文