Absorption of Horseradish Peroxidase (HRP) In Vitro Across Bovine Jejunal and Ileal Epithelia Around the Time of Weaning

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概要

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• Using the everted sac methodology as well as an Ussing chamber, we investigated changes in the absorption of horseradish peroxidase (EC 1.11.1.7, HRP (40 kDa)) in jejunum and ileum segments isolated from male Holstein cattle around the time of weaning (6 to 15 wks old). By the everted sac method, HRP transport (HRP concentration on the serosal side sampled after a 60-min-incubation) at 15 wks of age was significantly greater than that at 6 wks of age, in both segments of the intestine. Absorption was not significantly different between the jejunum and ileum. Addition of Na^+/K^+ ATPase inhibitor (ouabain, 1 mM) did not cause any significant change in HRP absorption, whereas Na^+/H^+ anti-transporter inhibitor (amiloride, 1μM) significantly increased the absorption in both sacs at 8 wks of age. By the Ussing chamber method, there were no significant differences between the values for Jsm and Jms, while the Jnet value was nearly zero for both epithelia. In addition, the flux (Jms) of Lucifer yellow, a cell-membrane-impermeable fluorescence dye, was significantly greater at 6 than at 13 wks of age in the ileal epithelia, although the flux was significantly greater in the jejunal than the ileal epithelia at both ages. From these findings, we conclude that: 1) bovine jejunal and ileal epithelia are able to absorb a large molecule such as the HRP protein; 2) HRP transport occurs in a concentration-dependent manner and may in part be via a paracellular pathway; 3) the increased HRP transport shown at 15 wks of age may not be caused by an increased use of the paracellular pathway.horseradish peroxidaseabsorptiontransportcalf

• 東北大学の論文
• 2005-11-25

著者

• KOBAYASHI Yosuke

  Department of Electrical Engineering, Tokai University graduate school

• OBARA Yoshiaki

  Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University

• Katoh Kazuo

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Obara Yoshiaki

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Katoh Kazuo

  Department Of Animal Physiology Faculty Of Agriculture Tohoku University

• NITTA Hayato

  Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University

• SUGAWARA Takuya

  Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University

• SUGAWARA Eri

  Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University

• Sugawara Takuya

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Sugawara Eri

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Kobayashi Yosuke

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Obara Yoshiaki

  Department Of Animal Husbundry Faculty Of Agriculture Tohoku University

• Nitta Hayato

  Department Of Animal Physiology Graduate School Of Agricultural Science Tohoku University

• Kobayashi Yosuke

  Department of Allergy and Respiratory Medicine, The Fraternity Memorial Hospital, Japan

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