Detection of Autoantibody to the 19kD Centromere Antigen Using Immunoblotting in Connective Tissue Diseases

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概要

• 論文の詳細を見る

• We tested sera from 388 patients including 113 with systemic sclerosis (SSc), 44 with sclerodactyly and Raynaud's phenomenon (SR), 100 with systemic lupus erythematosus (SLE), 47 with mixed connective tissue disease and 84 with primary Sjogren's syndrome (SS), for anticentromere antibodies (ACA) by immunoblotting, using nuclear-enriched sonicate of HeLa cells and affinity purified centromere antigen. Forty-one sera recognized a 19kD polypeptide when immunoblotted against both nuclear-enriched sonicate and purified antigen. ACA were also detected by immunofluorescence on chromosome spreads of Wil-2 cells in all these sera. But two of them did not show a discrete speckled pattern on immunofluorescence of Hep-2 cells due to masking phenomena with coexisting antinuclear antibodies (ANA). Three ACA positive sera failed to recognize the 19kD polypeptide. Six of the 44 sera with ACA contained coexisting other ANA (one with anti-topoisomerase I, two with anti-U_1RNP, two with anti-ENA that could not be further characterized and one with anti-dsDNA antibodies). ACA occasionally coexist with other ANA. So, immunoblotting analysis may be useful for the detection of ACA coexisting with other ANA. ACA were present in 16 (14%) of SSc, 19 (43%) of SR, 2 (2%) of SLE and 7 (8%) of primary SS. Raynaud's phenomenon was found in 43 (98%) of the ACA positive patients, though the other features of CREST syndrome (calcinosis, esophageal involvement, sclerodactyly and telangiectasia) were less frequently seen (range: 14%-52%). ACA may not be disease specific antibodies and are associated with Raynaud's phenomenon.

• 北里大学の論文
• 1992-02-29

著者

• Kondo Hirobumi

  Department of Rheumatology and Infectious Diseases, Kitasato University School of Medicine

• Kondo Hirobumi

  Departments Of Internal Medicine And Pathology Kitasato University School Of Medicine

• Kondo H

  Kitasato Univ. School Of Medicine

• Kondo Hirobumi

  Department Of Internal Medicine Kitasato Institute Medical Center Hospital

• KASHIWAZAKI Sadao

  Institute of Rheumatology, Tokyo Women's Medical College

• Kashiwazaki S

  Institute Of Rheumatology Tokyo Women's Medical College.

• Kashiwazaki Sadao

  Institute Of Rheumatology Tokyo Women's Medical College

• Takashina Naoya

  Department of Internal Medicine, Kitasato University School of Medicine.

• Takashina Naoya

  Department Of Internal Medicine Kitasato University School Of Medicine.

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