In situ酵素組織化学法によるラット脳及び肝Monoamine Oxidase活性の検討

概要

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In situ quantitative histochemical analysis on monoamine oxidase (MAO) activity in rat brain and liver was performed for its region specificity. Nitro blue tetrazolium was used for the formazan producing reductive reaction caused by enzymatic degradation product of tryptamine as MAO substrate. A linear relationship was obserbed between the integrated MAO activity and the absorbance analyzed by PC-associated image analysis. The MAO-A inhibitor, clorgylin, inhibited the MAO activity dose-dependently in brain region and liver tissue in vitro and in situ analyses with well correlation. These results suggested that this method could be useful for the quantitative analysis of MAO activity in tissue sections, especially in the rat locus coeruleus that plays an important role in noradrenergic neurons.
東北薬科大学の論文