ラットmonoamine oxidase mRNAの臓器分布と活性値との相関関係

概要

論文の詳細を見る
Monoamine oxidase A and B (MAO-A and -B, EC 1.4.3.4) are the major enzymes that catalyze the oxidative deamination of neurotransmitters, peripheral vasoactive amines and xenobiotics. MAO-A and -B can be distinguished by difference in substrate preference and inhibitor specificity, tissue and cell distribution, and immunological properties. There are significant discrepancies in the literature concerning the amount of MAO from a number of tissue sources. Using real-time PCR, we measured MAO mRNA expression levels and both amine oxidase activity without the influence of inhibitors. Then we compared tissue amine oxidase activity with the MAO-A and -B mRNA expression level by simple regression analysis. MAO-A mRNA expression level and benzylamine oxidative deamination activity showed good correlation. However, regression analysis of other substrates and mRNA levels showed moderate or no correlation. Further, we recalculated those data using multiple regression analysis. All substrates showed good or moderate correlation. In conclusion, these results indicated that there is a fixed relation of amine oxidase activity in tissues and mRNA expression level.
東北薬科大学の論文