新生児尿中硫酸抱合型胆汁酸の基準値設定と先天性胆道閉鎖症のスクリーニング検査開発に関する研究

概要

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It is well known that urinary sulfated bile acid (USBA) values increase in patients with cholestasis. Thus USBA can serve as an important marker of cholestasis, especially congenital atresia of bile duct which is hard to be diagnosed and has a high mortality rate. We developed a simple enzymatic assay to measure concentrations of USBA with use of bile acid sulfate sulfatase. We also evaluated this method in a screening assay for congenital atresia of bile duct. Concentrations of USBA were measured in samples of urine from 975 newborn babies at the 4th day and one month after birth. The values showed binomial distribution. We established the normal reference interval; the central 95% of results was 0.108 to 5.097 mmol/l at the 4th day and 0.095 to 5.136 mmol/l at one month after birth. The concentrations of USBA in patients with congenital atresia of bile duct were decreased significantly after one month treatment with UV radiation. This shows that urinary SBA can serve as an important diagnostic marker of congenital atresia in new born baby and also seems to be an indicator of cholestatic state in hepato-biliary diseases.
神戸大学の論文