Phagocytic Ability of Oyster Hemocytes Measured by a Simple Method Using Bacterial Thin-layer

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概要

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• A simple method called the phagocytic plaque assay was used to visually present and estimate the phagocytic ability of the hemocytes from the giant Pacific oyster, Crassostrea gigas, using adherent Staphylococcus aureus cells on a plastic dish. Many plaques appeared on the bacterial thin-layer when the oyster hemocytes were overlaid and incubated on killed cells of S. aureus. At both 20℃ and 30℃, the phagocytic ability of the oyster hemocytes were more activated with a significantly larger average area of plaques than were the hemocytes at 10℃. This method was considered to be practical and effective evaluating the qualification of phagocytosis by the oyster hemocytes, however, some problems for the quantification analysis still remain to be solved.

• 東北大学の論文
• 2005-04-25

著者

• Takahashi Keisuke

  Laboratory Of Aquacultural Biology Graduate School Of Agricultural Science Tohoku University

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