Application of Microwave Irradiation for Histochemical Analysis of Griffonia simplicifolia Agglutinin-1 (GSA-1) Binding Sites in Sigmo-rectal Polyps

概要

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Ten specimens each of carcinoma, adenoma and non-neoplastic mucosa which were obtained by endoscopic biopsy from sigmo-rectal polyps were treated with microwave irradiation and then examined by lectin histochemistry. The results were compared with those obtained by the conventional method. With the conventional method Griffonia simplicifolia Agglutinin-1 (GSA-l) binding sites on the apical surface were positively detected in 4/10 carcinomas but not in adenomas or non-neoplastic mucosa. In the Golgi area, the GSA-1 binding sites were revealed in 2/10 adenomas, but not in carcinomas or non-neoplastic mucosa. When microwave irradiation was applied, the number of GSA-1 binding sites detected on the apical surface increased to 7/10 carcinomas but remained negative in adenomas and non-neoplastic mucosa. In the Golgi area, detectable GSA-1 binding sites increased to 3/10 carcinomas, 9/10 adenomas and 9/10 non-neo-plastic mucosa. Comparing the conventional method with the microwave irradiation method, the incidences of the detectable GSA-1 binding sites increased through the microwave irradiation in both apical surface and Golgi area.
神戸大学の論文
1997-01-24