ラット大動脈移植モデルにおける組織学的検討

概要

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It is well known that human aortic allografts (including aortic valve) are finally complicated with degeneration and hyalinization of aortic walls. We investigated acute and chronic change in rat aortic allografts by histomorphological and immunohistological procedure. Aortic segments of Brown Norway rats were transplanted to Lewis rats as allografts. For control, aortic segments of Lewis rats were transplanted to Lewis rats as isografts. These rats were sacrificed at 9 days, 1, 3 and 6 months after transplantation. Paraffin sections of aortic segments were stained with hematoxylin and eosin for evaluation of morphological changes (thickness of the intima, thickness of the media, cell density of the media and cell density of the adventitia). Frozen sections were investigated by immunohistochemical staining. Intimal thickening has progressed from 1 month until 6 months after transplantation. The cell density and elastic fibers of the media were significantly decreased accompanied with the decrease in the thickness of the media. Anti-rat macrophage/monocyte antibody positive cells remarkably infiltrated to the adventitia in 9 days allografts. Our results indicated that antigen-antibody reactions in the adventitia contributed to the intimal hypertrophy in aortic allografts. Chronic changes in the media suggested fragility of aortic walls.
岐阜大学の論文
2003-03-31