食用菊中ガン細胞増殖抑制物質の単離と同定

概要

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A large number of data from epidemiologic and rodent studies has demonstrated that ingestion of vegetables and fruits is occasionally beneficial for reduction of cancer risks in humans. Thus, chemoprevention with food phytochemicals including minor dietary ingredients (food factors) is currently regarded as one of the new important scientific fields. Recently we found that the extract of chrysanthemum have anticancer effect as strong as that of tea flavonoid. In this paper we demonstrated the isolation and structural analysis of anticancer active substance from methanol extract of chrysanthemum. Potent cell growth suppressive compound designated D1 was isolated as major active component of chrysanthemum by successively chromatography on open column packed with ODS resin, and on 2 step reverse-phase HPLC in a Mightysil RP-18 column and a TSKgel ODS-80Ts column. The compound of D1 inhibits the proliferation of HL-60 cell distinctly (GI_<50> ; 19.3μg/ml). Examination by TOF-MS yielded a molecular weight of 539.1144 with a chemical composition C_<25>H_<24>O_<12>Na. By using ^1H, ^<13>C, NMR we have identified 3,5-d-o-caffeoylquinic acid as one of suppressive components on the growth of HL-60 cell.
八戸工業大学の論文
2004-02-27