Effect of Actin Filament on Deformation-Induced Ca^<2+> Response in Osteoblast-Like Cells

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概要

• 論文の詳細を見る

• Under the influence of mechanical environment, bone structure is formed and maintained by adaptive remodeling that involves osteoclastic resorption and osteoblastic formation. In the mechanotransduction system in osteoblasts, it is believed that intracellular calcium plays a fundamental role and cytoskeletal actin filament is a crucial component for the signal transduction process. To clarify the role of actin filament in deformation-induced Ca^<2+> signaling, osteoblast-like cells (MC3T3-E1) with different actin filament densities controlled by cytochalasin D were subjected to tensile strain in vitro. The change in intracellular Ca^<2+> concentration labeled by fluo-3 was observed using a confocal laser-scanning microscope. As a result, the disruption of the actin filament was found to significantly suppress the deformation-induced Ca^<2+> response that was regulated according to the degree of actin filament organization. This result indicates that the actin filament is indispensable for the quantitative regulation of Ca^<2+> signaling in response to a mechanical stimulus in osteoblasts.

• 一般社団法人日本機械学会の論文
• 2001-12-15

著者

• Tomita Yoshihiro

  Department Of Mechanical Engineering Faculty Of Engineering Kobe University

• Adachi Taiji

  Department Of Mechanical Engineering And Science Graduate School Of Engineering Kyoto University

• Hoshiai Sodai

  Graduate School Of Medical Tottori University

• Hoshiai Sodai

  Graduate School Of Science And Technology Kobe University

• MURAI Takayuki

  Nippon Steel Co. (Graduate School of Science and Technology, Kobe University)

• Murai Takayuki

  Nippon Steel Co. (graduate School Of Science And Technology Kobe University)

• Adachi Taiji

  Department of Biomechanics, Institute for Frontier Medical Sciences, Kyoto University:Department of Microengineering, Kyoto University

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• Preface
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