Local Disassembly of Actin Stress Fibers Induced by Selected Release of Intracellular Tension in Osteoblastic Cell

スポンサーリンク

概要

• 論文の詳細を見る

• In adherent cells, such as osteoblasts and endothelial cells, the actin stress fiber structure is dynamically reorganized under changes in the surrounding mechanical environment such as cyclic stretch deformation or extracellular fluid flow. Although many studies have been conducted to clarify the biochemical signaling pathways in the reorganization process, details of the reorganization mechanism are still not clearly understood. In addition, to known biochemical mediators, intracellular tension has been proposed as a candidate mechanical mediator for stress fiber reorganization. In previous studies, it was reported that the release of intracellular tension by contracting the cell body induced the disassembly of the stress fiber as an initial phase of the reorganization process. However, in these experimental systems, deformation or force was applied to the entire cell body, so that it was difficult to directly discuss the relationship between the individual mechanical condition of stress fibers and the disassembling phenomenon. In this study, we have designed a novel experimental system by which local contraction was applied to a single osteoblast-like cell and intracellular tension in the targeted stress fiber was selectively released. The dynamic change in stress fiber structure was observed in the EGFP-tagged actin expressed osteoblast-like cell MC3T3-E1. The results indicated that only tension-released stress fibers were selectively disassembled and disappeared in a single cell. This result suggests that the existence of intracellular tension is essential for the dynamical stability of the stress fibers in osteoblast-like cells.

• 一般社団法人 日本機械学会の論文

著者

• SAITO Naoaki

  Biosignal Research Center, Kobe University

• Tomita Yoshihiro

  Department Of Mechanical Engineering Faculty Of Engineering Kobe University

• Saito Naoaki

  Biosignal Research Center Kobe University

• Adachi Taiji

  Department of Biomechanics, Institute for Frontier Medical Sciences, Kyoto University:Department of Microengineering, Kyoto University

• SATO Katsuya

  Department of Applied Medical Engineering Science, Graduate School of Medicine, Yamaguchi University

• SHIRAI Yasuhito

  Biosignal Research Center, Kobe University

• TOMITA Yoshihiro

  Department of Mechanical and Systems Engineering, Graduate School of Science and Technology, Kobe University

関連論文

• Induction of Betacyanin Synthesis and Pigment Accumulation in Cell Suspension Cultures of Portulaca
• 1P-196 細胞接着パターニングによる細胞移動挙動の変化(細胞生物学的課題(1),第46回日本生物物理学会年会)
• ROLE OF MECHANICAL STRAIN IN THE MODULATION OF ACTIN STRUCTURE DYNAMICS IN MOTILE CELLS(1A3 Micro & Nano Biomechanics III)
• 1007 Calcium Response and Actin Structure Dynamics in Keratocytes Crawling on a Micropatterned Substrate
• Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration –Assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats–
• Tumor-Promoting Phorbol Esters Bind Directly to the Regulatory Domain of Diacylglycerol Kinase Gamma
• Two-Dimensional Quantitative Analysis of Preferential Alignment of BAp c-axis for Isolated Human Trabecular Bone Using Microbeam X-ray Diffractometer with a Transmission Optical System
• In-vitro assessment of fabric structural composite scaffold applicable to tissue regeneration around implants
• Effectiveness of scaffolds with pre-seeded mesenchymal stem cells in bone regeneration : Assessment of osteogenic ability of scaffolds implanted under the periosteum of the cranial bone of rats
• Isolation and Characterization of cDNAs Encoding an Enzyme with Glucosyltransferase Activity for cyclo-DOPA from Four O'clocks and Feather Cockscombs
• The Effects of Structure Orientation on the Growth of Fe_2B Boride by Multi-Phase-Field Simulation
• Three-Dimensional Lattice Continuum Model of Cancellous Bone for Structural and Remodeling Simulation
• Simulation of Trabecular Surface Remodeling based on Local Stress Nonuniformity
• Mechanical Remodeling of Bone Structure Considering Residual Stress
• Computational Simulation of Flow Localization Behavior
• NETWORK MODELS FOR GLASSY POLYMER AND PREDICTION OF INSTABILITY PROPAGATION
• Motion of a single particle in a horizontal pipe
• Effect of Particle-Particle Collision and Particle Rotation upon Floating Mechanism of Coarse Particles in Horizontal Pneurnatic Pipe
• 2P-009 張力がアクチンフィラメントの力学特性に及ぼす影響(蛋白質-構造,第47回日本生物物理学会年会)
• Preliminary Study on Mechanical Bone Remodeling Permitting Residual Stress
• Evaluation of Axial Strain in Stress Fibers Inducing Cytoskeletal Reorganization in Osteoblastic Cells(Micro- and Nano-biomechanics)
• Long-Term follow up of patients with conservatively treated Kienbock's disease
• Isolation of Stable, Variously Colored Callus Lines in Portulaca sp. : 'Jewel' and Analysis of Betalain Composition
• New Compo-Casting Method of Ceramics Inserted Cast Iron Reducing Thermal Stress
• Effect of Actin Filament on Deformation-Induced Ca^ Response in Osteoblast-Like Cells
• Multi-phase-field Simulations of Dynamic Recrystallization during Transient Deformation
• 1PT148 原子間力顕微鏡を用いたβ-カテニンの力学的挙動解析(日本生物物理学会第50回年会(2012年度))
• Mechanical Regulation of Actin Network Dynamics in Migrating Cells
• Local Disassembly of Actin Stress Fibers Induced by Selected Release of Intracellular Tension in Osteoblastic Cell
• Preface
• Thermal Dehydration and Decomposition of Dioxalato Complexes of Be(II), Mn(II), Co(II), Ni(II), Cu(II) and Zn(II) in Solid State
• Simulation Study on Local and Integral Mechanical Quantities at Single Trabecular Level as Candidates of Remodeling Stimuli

もっと見る 閉じる

スポンサーリンク