FUNCTION OF SYNDECAN-2 AS A RECEPTOR FOR FIBRONECTIN

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概要

• 論文の詳細を見る

• We have demonstrated an inverse correlation between the expression level of syndecan-2, a membrane spanning heparan sulfate proteoglycan, and metastatic potentials of the tumor cells cloned from mouse Lewis lung carcinoma 3LL, and confirmed a causal relation between the two phenotypes using the cells manipulated syndecan-2-expression. Syndecan-2 acts as a receptor for fibronectin in vitro in cooperation with integrin α5β1 and the difference of its expression level resulted in the induction of different types of actin cytoskeletal organization on cell adhesion to a fibronectin substratum. Despite the similar expression levels of integrin α5β1 and other members of a syndecan family between the two clones, low metastatic P29 cells with high expression of syndecan-2 formed stress fibers on fibronectin although highly metastatic H11 cells with low expression of syndecan-2 induced cortex actin formation. Moreover, P29 cells suppressed syndecan-2 expression with antisense oligonucleotide formed cortex actin and H11 cells elevated syndecan-2 expression by transfection with cDNA (H11-SN2 cells) formed stress fibers on fibronectin, confirming that syndecan-2 modulates ligand-binding integrin α5β1 signaling in expression level-dependent manner. Syndecan-2 bound to Hep-II domain of fibronectin with its heparan sulfate side chains by a [IdoA(2S)α1→4GlcNS(6S)]_6 structure. The immobilized antibody against to the ectodomain of syndecan-2 resulted in the similar effect as the immobilized Hep-II domain to induce stress fiber formation in P29 cells, indicating that clustering of syndecan-2 through either heparan sulfate side chains or core proteins generates the same signal. Interestingly, antisyndecan-4 antibody also induced stress fiber formation in P29 cells but not in H11 cells which expressed the same level of syndecan-4 to P29 cells, suggesting that syndecan-2 acts at downstream of syndecan-4 in the cascade. We will discuss the cross talking of syndecan-2 and -4 involved in the signaling for actin cytoskeletal organization.

• 日本結合組織学会の論文

著者

• Hirose Mayumi

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Hirose Mayumi

  Department Of Bioregulation Biomedical Research Center Osaka University Graduate School Of Medicine

• Yamashina Ikuo

  Department Of Biotechnology Faculty Of Engineering

• MUNESUE Seiichi

  Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University

• Oguri Kayoko

  Clinical Research Center National Hospital Organization Nagoya Medical Center

• Oguri Kayoko

  Clinical Research Center Nagoya National Hospital

• 岡山 實

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Okayama Minoru

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• 棟居 聖一

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Kusano Yuri

  Clinical Research Institute, National Nagoya Hospital

• Yoshitomi Yasuo

  Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University

• 棟居 聖一

  京都産業大学工学部事務室

• Kusano Yuri

  Clinical Research Center National Hospital Organization Nagoya Medical Center

• Yoshitomi Yasuo

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Munesue Seiichi

  Department Of Biochemistry And Molecular Vascular Biology Kanazawa University Graduate School Of Med

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• FUNCTION OF SYNDECAN-2 AS A RECEPTOR FOR FIBRONECTIN
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