Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme.
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概要
- 論文の詳細を見る
The cloned cDNA for batroxobin has been expressed in E. coli. Batroxobin could only be obtained as intracellular aggregates of fusion proteins, fused with a small peptide. To obtain the mature batroxobin, the recognition sequence for thrombin was inserted between the peptide and the mature batroxobin. This fusion protein accumulated in an insoluble form and could easily be purified. After site-specific cleavage of the fusion protein with thrombin, recombinant batroxobin was isolated by preparative electrophoresis. Batroxobin with enzymatic activity was obtained by the refolding of recombinant batroxobin.
- 社団法人 日本生化学会の論文
著者
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Yamashina Ikuo
Department Of Biotechnology Faculty Of Engineering
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Itoh Nobuyuki
Department Of Chemistry Science University Of Tokyo
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Niwa Mineo
Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd.
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Maeda Masahiro
Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd.
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Satoh Susumu
Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd.
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Suzuki Shingo
Product Development Laboratories, Fujisawa Pharmaceutical Co., Ltd.
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- Expression of cDNA for batroxobin, a thrombin-like snake venom enzyme.