Cooperation of Syndecan-2 and Syndecan-4 among Cell Surface Heparan Sulfate Proteoglycans in the Actin Cytoskeletal Organization of Lewis Lung Carcinoma Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Syndecan-2 cooperates with integrin a5pl in cell adhesion to a fibronectin substratum and regulates actin cytoskeletal organization in an expression level-dependent manner; Lewis lung carcinoma-derived P29 cells with high expression form stress fibers, whereas the same tumor-derived low expressers, LM66-H11 cells, form cortex actin [Munesue, S., Kusano, Y., Oguri, K., Itano, N., Yoshitomi, Y., Nakanishi, H., Yamashina, I., and Okayama, M. (2002) Biochem. J. 363, 201-209]. In this study we examined the participation of other cell surface heparan sulfate proteoglycans in this signaling. The two clones expressed syndecan-1, -2 and -4, and glypican-1 at similar levels except for syndecan-2. Treatment of cells with phosphatidylinositol-specific phospholipase C or immobilized anti-syndecan-1 antibodies demonstrated that neither glypican-1 nor syndecan-1 was involved in this signaling, indicating that individual cell surface heparan sulfate proteoglycans have functional specificity. Stimulation with immobilized anti-syndecan-2 or -4 antibodies induced stress fiber formation in P29 cells but not in LM66-Hll cells, despite the similar levels of syndecan-4 expression, suggesting that stress fiber formation required a threshold expression level of syndecan-2 acting downstream of syndecan-4. This was confirmed by cells in which syndecan-2 expression was artificially suppressed by antisense mRNA oligonucleotide treatment or elevated by cDNA transfection. This is the first report demonstrating that syndecan-2 and -4 cooperate in situ in actin cytoskeletal organization.

• 社団法人 日本生化学会の論文

著者

• MUNESUE Seiichi

  Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University

• Oguri Kayoko

  Clinical Research Center Nagoya National Hospital

• Okayama Minoru

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Kusano Yuri

  Clinical Research Center National Hospital Organization Nagoya Medical Center

• Yoshitomi Yasuo

  Department Of Biotechnology Faculty Of Engineering Kyoto Sangyo University

• Munesue Seiichi

  Department Of Biochemistry And Molecular Vascular Biology Kanazawa University Graduate School Of Med

関連論文

• Identification of the Core Protein Carrying the Tn Antigen in Mouse Brain: Specific Expression on Syndecan-3
• Difference in Laminin Expression Between High and Low Metastatic Cell Clones Derived from Murine Lewis Lung Carcinoma
• Regulation of alternative splicing of the receptor for advanced glycation endproducts (RAGE) through G-rich cis-elements and heterogenous nuclear ribonucleoprotein H
• FUNCTION OF SYNDECAN-2 AS A RECEPTOR FOR FIBRONECTIN
• OVEREXPRESSION OF SYNDECAN-2 SUPPRESSES THE METASTATIC PHENOTYPE OF MOUSE LEWIS LUNG CARCINOMA CELLS
• Matrix Metalloproteinase-9 Associated with Heparan Sulphate Chains of GPI-Anchored Cell Surface Proteoglycans Mediates Motility of Murine Colon Adenocarcinoma Cells
• Cooperation of Syndecan-2 and Syndecan-4 among Cell Surface Heparan Sulfate Proteoglycans in the Actin Cytoskeletal Organization of Lewis Lung Carcinoma Cells
• Involvement of Syndecans in Actin Cytoskeletal Organization and Tumor Metastasis
• シンデカンの機能特異性
• Can syndecan-1 become a prognostic factor in solid tumors?
• Cooperation of Syndecan-2 and Syndecan-4 among Cell Surface Heparan Sulfate Proteoglycans in the Actin Cytoskeletal Organization of Lewis Lung Carcinoma Cells

もっと見る 閉じる

スポンサーリンク