新しい除草剤作用検定法としての種子根伸長テスト

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Studies were carried out to develop a new bio-assay method for evaluating the effects of chemicals on root elongation. In the procedure, a polyethylene disc 5 cm in diameter and 0.3 cm thick on which germinated plant seeds had been placed was floated on water in the presence or absence of chemicals (Fig. 1) ; and subsequently the root elongation was observed. The results obtained are as follows: (1) Rice plant (cv. Akinishiki) was found to be the most suitable species for the bioassay among 7 gramineous plants tested, since the species showed a uniform growth and the fasted elongation of seminal roots in water (Fig. 2). (2) Growth of the rice seminal roots was markedly affected by the changes in the light and temperature conditions. It was demonstrated that incubation in the dark at 30℃ was optimum to secure uniform and rapid elongation of the roots (Tables 1 and 2). (3) Maximum elongation rate (3.8 cm/day) of the roots was obtained on the third day of the incubation (Fig. 3). (4) Effects of organic solvents, surfactants and a carrier of herbicidal wettable powder on the elongation of the roots were investigated by adopting the new method under the conditions, previously descrived. Ethanol and the carrier did not inhibit the elongation of the rice seminal roots at concentrations ranging from 0.1 to 300 ppm (w/w) (Fig. 4). (5) The elongation of the rice seminal roots cultured by the new method was compared to that of roots cultured in water in a petri-dish. It was evident that the former roots grew faster than the latter without twisting, hence evaluating precise measurements of the root length (Fig. 5 and Photo 1). (6) Effect of pretilachlor [2-chloro-2', 6'-diethyl-N-(2-propoxyethyl) acetanilide] on the elongation of the rice seminal root and safening effects of CGA-123,407 [4, 6-dichloro-2-phenylpyridine] as well as NA on the herbicide could be clearly evaluated through this bioassay method (Fig. 6 and Fig. 7). The result obtained suggest that this method could be applied for investigating the herbicidal action of chemicals on root elongation.
日本雑草学会の論文
1990-07-27

新しい除草剤作用検定法としての種子根伸長テスト

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