Construction of a Nanosecond Fluorometric System for Applications to Biological Samples at Cell or Tissue Levels
スポンサーリンク
概要
- 論文の詳細を見る
A fluorometric system utilizing the single photon counting technique which records the decay of the intensity and the anisotropy of fluorescence following pulse excitation is described. Both macroscopic observations on cellular suspensions and measurements on individual cells under an optical microscope are possible. The system is so designed to permit precise evaluation of complex anisotropy decays, which is essential for studies of cells or tissues. Data are processed by a method which properly analyzes these complex decays. A preliminary study on erythrocyte membrane has confirmed the high resolution and precision of the system.
- 社団法人応用物理学会の論文
- 1976-12-05
著者
-
Mitaku Shigeki
Department Of Applied Physics Faculty Of Engineering University Of Tokyo:(present Address) Departmen
-
Kinosita Kazuhiko
Department Of Physics Faculty Of Science And Engineering Waseda University
-
Kinosita Kazuhiko
Department Of Physics Faculty Of Science University Of Tokyo:(present Address) Department Of Physiol
-
Ohbo Nobuo
Department Of Information Science Faculty Of Science University Of Tokyo
-
Ikegami Akira
Department Of Physics Faculty Of Science University Of Tokyo
-
KUNII Toshiyasu
Department of Information Science, Faculty of Science, University of Tokyo
-
Kunii Toshiyasu
Department Of Information Science Faculty Of Science University Of Tokyo
-
Mitaku Shigeki
Department Of Applied Physics Faculty Of Engineering University Of Tokyo
-
Ikegami Akira
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
関連論文
- 3P-096 紫膜中のバクテリオロドプシンの光退色による構造変化(膜蛋白質,第46回日本生物物理学会年会)
- Structural Change of Bacteriorhodopsin in the Purple Membrane above pH 10 Decreases Heterogeneity of the Irreversible Photobleaching Components
- 3P178 リバースジャイレースの温度依存性(分子モーター,第48回日本生物物理学会年会)
- 3P093 AFM single-molecule force spectroscopy of bacteriorhodopsin in delipidated purple membrane(Protein: Measurement & Analysis,The 48th Annual Meeting of the Biophysical Society of Japan)
- 2P298 グラム陰性菌中のタンパク質の細胞内局在性でのゲノムスケール解析(生命情報科学-比較ゲノミクス,第48回日本生物物理学会年会)
- 2P300 コイルドコイル予測システムSOSUIcoilを用いた生物ゲノム比較(生命情報科学-比較ゲノミクス,第48回日本生物物理学会年会)
- Regeneration of Bacteriorhodopsin from Thermally Unfolded Bacterio-Opsin and All-trans Retinal at High Temperatures
- 2P299 正の電荷相関を持つタンパク質によるゲノム解析(生命情報科学-比較ゲノミクス,第48回日本生物物理学会年会)
- 3P025 原子間力顕微鏡観測による紫膜のBumpsの解析(蛋白質-構造,第48回日本生物物理学会年会)
- 3P060 AUF指標と疎水性指標を用いたアミロイド形成タンパク質の配列情報解析(蛋白質-物性(安定性,折れたたみなど),第48回日本生物物理学会年会)
- Heterogeneity in Regeneration of Bacteriorhodopsin from Bacterio-Opsin and All-trans Retinal at High Temperatures : Implications for Dynamic Structural Fluctuations
- Spectral Analysis of Amino Acid Sequence.I.Intrinsic Membrane Proteins
- 3TP3-02 II型DNAトポイソメラーゼの手品のような反応を直接みる(バイオイメージング,第47回日本生物物理学会年会)
- 3P-261 II型DNAトポイソメラーゼの手品のような反応を直接みる(バイオイメージング,口頭発表,第47回日本生物物理学会年会)
- 2P118 化学ポテンシャルに基づいたバクテリオロドプシンのフォースカーブのシミュレーション(膜蛋白質,第48回日本生物物理学会年会)
- 3P-085 DMPC、DPPC二層膜に再構成したバクテリオロドプシンの光退色(膜蛋白質,第47回日本生物物理学会年会)
- 3P-084 紫膜バクテリオロドプシンの光退色現象におけるタンパク質-脂質間相互作用の影響(膜蛋白質,第47回日本生物物理学会年会)
- 3P-230 物理化学的性質の解析による膜タンパク質のトポロジー予測法の開発(生命情報科学-構造ゲノミクス,第47回日本生物物理学会年会)
- 1P-091 バクテリオロドプシンのフォースカーブのシミュレーション(膜蛋白質,第47回日本生物物理学会年会)
- Effect of Molecular Assembly on Photocycle of Reconstituted Bacteriorhodopsin : Significant Blue Shift of the Late M Photointermediate in the Liquid Crystalline Phase
- Visualization of RecA Filaments and DNA by Fluorescence Microscopy
- Transient and Steady-State Deformations of a Vesicle with an Insulating Membrane in Response to Step-Function or Alternating Electric Fields
- Deformation of Vesicles under the Influence of Strong Electric Fields II
- Deformation of Vesicles under the Influence of Strong Electric Fields
- Vertebrate Genomes Code Excess Proteins with Charge Periodicity of 28 Residues
- 1P469 Characteristic cellular localization of proteins which have charge periodicity of 28 residues(23. Bioinformatics, genomics and proteomics (I),Poster Session,Abstract,Meeting Program of EABS &BSJ 2006)
- SEM Imaging of Single Heavy Meromyosin Molecules on Hydrophilic Silicon Surface
- Ultrasonic Resonance Spectrometry with Fourier Synthesized Pseudorandom Noise Excitation and Its Application to a Lyotropiec Liquid Crystal
- Spectral Analysis of Amino Acid Sequence.II.Characterization of α-Helices by Local Periodicity
- Studies of Ordered Monodisperse Latexes. : IV. Mechanism of Ordering
- Studies of Ordered Monodisperse Latexes. : III. Viscoelasticity and Flow Properties
- Studies of Ordered Monodisperse Polystyrene Latexes. : I. Shear Ultrasonic Measurements
- Thermotropic Behavior of Membranes Containing DMPC and Chemically and Stereochemically Pure Sphingomyelin upon Cholesterol Addition(Cross-disciplinary Physics and Related Areas of Science and Technology)
- Ultrasonic Properties of Melittin Solution : Physical Acoustics
- Ultrasonic Properties of Proteins : Physical Acoustics
- 2P162 Kinetic Study for Irreversible Photobleaching of Bacteriorhodopsin in Purple Membrane at Alkaline pH(34. Membrane protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Irreversible Photobleaching of Bacteriorhodopsin in a High-Temperature Intermediate State
- Reconstitution of Bacteriorhodopsin into Cyclic Lipid Vesicles
- Ultrasonic Measurement of Bacteriorhodopsin in Purple Membrane : Physical Acoustics
- Construction of a Nanosecond Fluorometric System for Applications to Biological Samples at Cell or Tissue Levels
- Measurements of Ultrasonic Velocity of Erythrocyte Ghosts in Suspension with Sing-Around Method
- 1P377 Discrimination of G-protein by analyzing physicochemical properties of N- and C- terminal regions of G-proteins(15. Cellular signal transduction,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Mechanisms of Secondary Structure Breakers in Soluble Proteins
- Cooperative Association of Actin Protomers and Crosslinked Actin Oligomers in Filaments at Low Ionic Strength^1
- 2P225 Torque and Potential measurement of F1-ATPase(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- Characteristic amino acid distribution around segments unique to allergens
- Ultrasonic Measurement of Melittin-Liposome System : Physical Acoustics
- Ultrasonic Spectrometer for Solutions of Biological Meterial : Medical Ultrasonics and Ultrasonic Measurements
- 2P223 Unidirectional Orientation of Actin Filaments(37. Molecular motor (II),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 3I1024 Bumps on delipidated purple membrane analyzed by AFM(3I Protein: Measurement & Analysis 1,The 49th Annual Meeting of the Biophysical Society of Japan)
- 3B1510 Temperature dependence of positive supercoiling by reverse gyrase(3B Nucleic acid binding proteins,The 49th Annual Meeting of the Biophysical Society of Japan)
- Ratio of membrane proteins in total proteomes of prokaryota
- Human Genome Encodes Many Proteins with Charge Periodicity of 28 Residues
- Electrostatic effects influence the formation of two-dimensional crystals of bacteriorhodopsin reconstituted into dimyristoylphosphatidylcholine membranes
- Physicochemical Properties of Starches Isolated from Five Cassava (Manihot esculenta Crantz) Landraces of Brazil
- 3PT120 紫膜表面の隆起構造およびバクテリオロドプシン分子構造に与える紫膜中の古細菌型脂質除去の影響(日本生物物理学会第50回年会(2012年度))
- Fluctuation of the Myoglobin Structure
- 2P107 紫膜表面において観測される隆起構造体の曲率に対する溶媒pHやイオン強度の影響(03.膜蛋白質,ポスター,日本生物物理学会年会第51回(2013年度))
- Cooperative Association of Actin Protomers and Crosslinked Actin Oligomers in Filaments at Low Ionic Strength.