ヒトスジシマカにおけるチクングニアウイルス量の螢光抗体法による測定の試み

概要

論文の詳細を見る
A focus counting method using the indirect fluorescent antibody technique was adopted to demonstrate the multiplication of chikungunya virus in individual Aedes albopictus mosquito of large numbers. The number of foci was counted 20hr after the inoculation of test samples into BHK-21 cells and the amount of virus was expressed semiquantitatively as -, +, 2+, 3+ and 4+. The grade of foci was proportional to the results of plaque assay. Most of test specimens with confluent foci (4+) showed (10)^6-(10)^7 PFU/mosquito. Marked differences were observed in the amount of virus in mosquitoes among different strains. percentages of individuals showing 4+ grade foci were 30.0% in Oahu strain and 5.0% in Osaka strain. no high-grade individual was observed in the strains from okinawa, Sendai, Taiwan, Thailand and Indonesia. Rearing conditions for adult mosquitoes tended to affect the occurrence of the high-grade individuals.
日本衛生動物学会の論文
1986-12-15