Studies on ribonucleic acids from Chlorella protothecoides with special reference to the degradation of chloroplast RNA during the process of "glucose-bleaching"
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概要
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By growing Chlorella protothecoides under certain nutritional and light conditions the following three different types of algal cells were obtained: (i) normal "green" cells grown in a medium rich in a nitrogen source (urea) and poor in glucose under illumination, (ii) "etiolated" cells cultivated in the same medium in darkness, and (iii) "glucose-bleached" cells grown, in the light or in darkness, in a medium rich in glucose and poor in the nitrogen source. The "glucose-bleached" cells contain profoundly degenelated plastids and the "etiolated" cells have only partially organized plastids. From these algal cells RNA was extracted by the cold phenol method, and fractionated by MAK column chromatography and sucrose density gradient centrifugation, making use of ^<32>P-labelled E. coli RNA as the internal marker. It was found that in comparison with the green cells that are rich in chloroplast ribosomal RNA as well as in non-chloroplastic ("cytoplasmic") ribosomal RNA, the etiolated cells possess a comparable amount of "cytoplasmic" rRNA but a significantly lesser amount of chloroplast rRNA. Both types of rRNA exist at extremely low levels in the glucose-bleached cells. During the process of bleaching (chloroplast degeneration) of the green cells induced by the addition of a high concentration of glucose, marked changes were observed in the patterns of fractionation of RNA as followed by the above procedures. It was disclosed that the chloroplast rRNA is rapidly degraded during an early phase of the bleaching process, while the quantity of "cytoplasmic" rRNA remained almost unaltered.
- 日本植物生理学会の論文
著者
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Hase Eiji
Tokugawa Institute For Biological Research
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Mihara Sayoko
Institute Of Applied Microbiology University Of Tokyo
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KIMURA KOICHI
Institute of Applied Microbiology, University of Tokyo
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Kimura Koichi
Institute Of Applied Microbiology University Of Tokyo:(present)faculty Of Pharmaceutical Sciences Un
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