cDNA Cloning and Tissue Specific Expression of a Gene for Sucrose Transporter from Rice (Oryza sativa L.)
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概要
- 論文の詳細を見る
We describe the cloning and expression analysis of a sucrose transporter cDNA from a monocot (the rice plant, Oryza sativa L.). The cDNA clone (OsSUT1) encoded an open reading frame of 1,611 bp (537 amino acids) and showed 76.8 to 79.7% Similarity at the amino acid level to other sucrose transporters of dicot species. The predicted membrane topology of OsSUT1 protein is made up of 12 transmembrane helices which is consistent with most of the mono- and disaccharide transporters previously identified. When OsSUT1 cDNA was introduced into yeast and expressed, the cells rapidly accumulated sucrose demonstrating that OsSUT1 does, in fact, encode a sucrose transporter. From genomic Southern hybridization OsSUT1 appeared to be a single copy gene. OsSUT1 was expressed in source organs such as leaf blade, leaf sheath and germinating seed whereas little or no expression was observed in some sink organs such as the panicles before heading and the roots. Transcript was observed at high levels in panicles after heading, particularly in the portion containing endosperm and embryo. In addition, expression of OsSUT1 was high in etiolated seedlings and decreased during light-induced greening.
- 日本植物生理学会の論文
著者
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IMAIZUMI Nobuyuki
Japan Turfgrass Inc.
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Ohsugi R
National Institute Of Agrobiological Sciences:(present Office) Graduate School Of Agricultural And L
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Ohsugi Ryu
National Institute Of Agrobiological Resources
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Ohsugi Ryu
Department Of Agricultural And Environmental Biology Graduate School Of Agricultural And Life Scienc
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Ohsugi Ryu
Niar
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Hirose Tatsuro
Department of Plant Physiology, National Institute of Agrobiological Resources
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Scofield Graham
CSIRO Division of Plant Industry
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Furbank Robert
CSIRO Division of Plant Industry
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Hirose Tatsuro
Hokuriku National Agricultural Experiment Station
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Furbank Robert
Csiro Division Of Plant Industry:co-operative Research Centre For Plant Science Research School For
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Scofield Graham
Csiro Division Of Plant Industry:co-operative Research Centre For Plant Science Research School For
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Imaizumi N
Japan Turfgrass
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Imaizumi Nobuyuki
Japan Turfgrass
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