Differential Exposure of Tryptophan Residues in the Red and Far-Red Light Absorbing forms of Phytochrome, as Revealed by Chemical Modification

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概要

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• The effects of the chemical modification of tryptophan residues in native pea (Pisum sativum L.) phytochrome by 2-hydroxy-5-nitrobenzyl bromide (HNB-Br) were examined. Such treatment had no effect on the spectral properties or on the pattern of tryptic digestion of phytochrome, which indicated that no major conformational change in phytochrome had occurred. Amino acid analysis of the HNB-Br-treated phytochrome indicated that the number of modified Trp residues after the treatment was dependent on the light-absorbing form. The values were three for P_R and five for P_<FR> (out of a total of ten) per monomer. The results indicate that two additional Trp residues are exposed on the molecular surface of P_<FR> when the photoconversion of P_R to P_<FR> occurs. The amino acid analysis of a 58-kDa tryptic fragment of phytochrome (a mixture of peptides, residues 63-583 and 66-587) showed that one Trp residue in the fragment from P_R and two in that from P_<FR> (out of six) were modified by HNB-Br. In the 56-kDa fragment (a mixture of peptides, residues 598-1121 and 603-1124), there were two modified Trp residues in P_R and three in P_<FR> (out of four). The Trp residue in a 36-kDa fragment (residues 66-383), which includes the tetrapyrrolic chromophore, was not modified in the either case. These results indicate that new exposed sites that are generated by the photoconversion of P_R to P_<FR> are in the region between Trp-456 and Trp-567 and in that between Trp-644 and Trp-787.

• 日本植物生理学会の論文

著者

• Nakazawa Miki

  Department Of Biology Yokohama City University

• Manabe Katsushi

  Department Of Applied Physics Tokyo Institute Of Technology:(present Address) Railway Technical Rese

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• Differential Exposure of Tryptophan Residues in the Red and Far-Red Light Absorbing forms of Phytochrome, as Revealed by Chemical Modification
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