Low Temperature Spectrophotometry on Phototransformation of P_R in Pelletable Phytochrome of Pea

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Phototransformation of P_R to P_<FR> m a 1,000-7,000 ×g pelletable fraction (1-7 KP), which was extracted from dark-grown pea shoots that had been irradiated by red then far-red light, was studied by low temperature spectrophotometry. Red light irradiation of P_R in 1-7 KP at - 160℃ induced an absorption increase at 695-696 nm with a concomitant, small decrease of P_R absorption at 670 nm. These changes were partially photoreversed when the sample was irradiated subsequently with 700-nm light. At -55℃, red light irradiation of P_R resulted mainly in bleaching and consequently in a reduction of the P_R Peak, accompanied by minor absorbance increases around 695 nm. The inter-mediates formed at -165℃ by 660-nm light irradiation partly reverted back to P_R or formed a bleached intermediate (probably the same bleached intermediate described above) in the dark, when the pellets were warmed to -60℃. The bleached inter-mediate was transformed to P_<FR> in the dark at -10℃ or above. These characteristics of P_R transformation observed in the pelletable phytochrome were essentially the same as those observed in in vivo or soluble phytochrome.
日本植物生理学会の論文