Phytochrome in the Fern, Adiantum capillus-veneris L.: Spectrophotometric Detection in Vivo and Partial Purification

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Spectrophotometric studies of fern phytochrome were performed using dark-grown leaves of Adiantum. The absorbance difference spectrum between the red- and far-red-light irradiated sample showed a photoreversible absorbance change in the far-red region, with a maximum located at 728-730 nm. The concentration of phytochrome was highest at the leaf tips and decreased gradually along the leaf axis. As in the case of angiosperm phytochrome, the level of fern phytochrome decreased under continuous white light, and the level increased again when de-etiolated tissue was transferred back to the dark. When the fern tissue was exposed to a pulse of red light, the dark reversion of P_<FR> to P_R took place with almost no destruction of P_<FR>. Phytochrome could be extracted from light-grown young leaves of the fern with a slightly alkaline, aqueous buffer that contained 1 M NaCl. The difference spectrum of the partially purified phytochrome from fern was similar to that of partially degraded phytochrome from angiosperms. A polyclonal antibody raised against phytochrome from etiolated rye seedlings immunostained (albeit weakly) a 110-kDa polypeptide after fractionation by SDS-polyacrylamide gel electrophoresis of the preparation of fern phytochrome. The band was very probably fern phytochrome since it emitted zinc-induced fluorescence.
日本植物生理学会の論文