Re-Formation of the Preprophase Band After Cold-Induced Depolymerization of Microtubules in Adiantum Protonemata

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Re-formation of the preprophase band (PPB) of microtubules (MTs) after cold-induced depolymerization of MTs was investigated in protonemal cells of the fern, Adiantum capillusveneris L. When protonemata with a PPB were chilled at O℃, MTs of the PPB depolymerized within 5 min leaving only a few fragments of MTs, and all such fragments were disrupted within 2 h after the onset of cold treatment. When cells were chilled for 5 min and then rewarmed at 25℃, the transverse MTs of the PPB gradually increased in number in the region of the PPB and re-formation of the PPB was accomplished within 20 min. In contrast, when cells chilled for 2 h were rewarmed, randomly oriented MTs appeared initially and then a PPB with a low density of MTs became apparent. The PPB re-formed even when the nucleus and most of the endoplasm had been displaced from the region of the PPB by centrifugation (2,800×g, 15 min). These results suggest that MTs of the PPB nucleate and are organized in the cortical cytoplasm during re-formation of the PPB after disruption.
日本植物生理学会の論文