Effects of Blue Light and Ammonia on Nitrogen Metabolism in a Colorless Mutant of Chlorella

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概要

• 論文の詳細を見る

• Illumination of a colorless mutant of Chlorella vulgaris 11h (M125) with blue light enhanced both the uptake of nitrate and the release of ammonia. These effects were not observed under illumination with red light. The release of ammonia was also enhanced by the addition of methionine sulphoximine (MSX), an inhibitor of glutamine synthetase (GS). Addition of MSX to cultures in the dark increased the rate of breakdown of starch. Algal cells grown in nitrate-containing medium did not show the aminating activity of glutamate dehydrogenase (GDH). Addition of large (millimolar) amounts of ammonia in the dark resulted in the induction of NADPH-GDH activity and, in addition, a decrease in GS activity. From these results it appears that GS catalyzes the primary step in the assimilation of ammonia in algal cells grown in nitrate-containing medium. Two isoforms (GS_1 and GS_2) of GS have been separated by ion exchange chromatography. The activities of both isoforms were decreased upon the addition of ammonia. Illumination of the alga with blue light at intensities up to 10,000 mW・m^<-2> enhanced the measurable activity of GS in vitro, while higher intensities were ineffective. In red light no such effect was observed. The effects of blue light and ammonia on nitrogen metabolism in algal cells are discussed.

• 日本植物生理学会の論文

著者

• Kamiya Akio

  Laboratory Of Chemistry Faculty Of Pharmaceutical Sciences Teikyo University

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