Auxin-Binding Protein in Etiolated Mung Bean Seedlings : Purification and Properties of Auxin-Binding Protein-II

スポンサーリンク

概要

• 論文の詳細を見る

• An auxin-binding protein (ABP-II) was purified from the extract of etiolated mung bean seedlings by affinity chromatography on 2,4-D-linked Sepharose 4B and by gel filtration on Sepharose 4B and Sephacryl S-200. The molecular weight was estimated to be about 190,000 by gel filtration on Sephacryl S-200. ABP-II gave a single band corresponding to a molecular weight of about 48,000 on SDS-polyacrylamide gel electrophoresis. The dissociation constants of ABP-II for 2,4-D determined by ammonium sulfate precipitation and equilibrium dialysis were 9.5 × 10^<-6> M and 1.1 × 10^<-5> M, respectively. ^<14>C-2,4-D-binding to ABP-II was reversible and inhibited by addition of IAA, naphthalene-1-acetic acid, 2,4,5-trichlorophenoxyacetic acid or p-chlorophenoxyisobutylic acid to the assay mixture.

• 日本植物生理学会の論文

著者

• Sakai S

  Institute Of Biological Sciences University Of Tsukuba

• Sakai Shingo

  Laboratory of Cell Biochemistry, Faculty of Science, Saitama University

• Sakai Shingo

  Laboralory Of Cell Biochemistry Faculty Of Science Saitama University

関連論文

• Modulation of Gene Expression in Isolated Nuclei by Auxin-Binding Proteins
• Stimulation of RNA Synthesis in Isolated Nuclei by Auxin-Binding Proteins-I and -II
• Cloning of a cDNA for a 1-Aminocyclopropane-1-Carboxylate Synthase That Is Expressed during Development of Female Flowers at the Apices of Cucumis sativus L.
• AN ACC SYNTHASE GENE POSSIBLY INVOLVEDIN THE FORMATION OF FEMALE FLOWERS IN CUCUMBER PLANTS
• Auxin-Binding Protein in Etiolated Mung Bean Seedlings : Purification and Properties of Auxin-Binding Protein-II
• Purification of an Auxin-Binding Protein from Etiolated Mung Bean Seedlings by Affinity Chromatography
• Biosynthesis of ethylene in sweet potato root tissue
• Effects of the Inhibitory Protein of Ethylene Synthesis on ATP Levels in Mung Bean Hypocotyl Segments
• Characterization of 2, 4-D Binding to the Auxin-binding Protein Purified from Etiolated MungBean Seedlings

スポンサーリンク