Affinity chromatography of an ethylene-synthesizing enzyme from red alga Porphyra tenera on an immobilized inhibitor of ethylene evolution
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概要
- 論文の詳細を見る
A method was established to purify acrylate decarboxylase from Porphyra tenera by affinity chromatography using a proteinaceous inhibitor of ethylene evolution in marine algae, isolated from P. tenera as a ligand. The proteinaceous inhibitor was covalently coupled to porous glass via four different spacer arms. The porous glass-aminopropyltriethoxysilane-succinatephenylendiamine-succinate-inhibitor appeared to be the best derivative for retaining acrylate decarboxylase extracted from P. tenera. Acrylate decarboxylase was extracted from 10 kg of P. tenera and semi-purified by ammounium sulfate preparation and gel filtration on Sephadex G-100. The active fraction was applied to an affinity column. Acrylate decarboxylase was eluted in the starting buffer containing 0.2 M NaCl. Ethylene formation from acrylate was detected in the presence of this enzyme extract, but not in the case of the boiled enzyme extract. Acrylate decarboxylase was inhibited by the inhibitor isolated from P. tenera. These facts indicate that the formation of ethylene in marine algae from acrylate proceeds enzymatically.
- 日本植物生理学会の論文
著者
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FUJII Tadashi
Department of Electronic Science and Engineering, Faculty of Engineering, Kyoto University
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Noguchi Teruhisa
Laboratory Of Biochemistry Teijin Institute For Biomedical Research Teijin Limited
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Watanabe Tsuneo
Laboratory of Biochemistry, Teijin Institute for Biomedical Research, Teijin Limited
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Kondo Noriaki
Laboratory of Biochemistry, Teijin Institute for Biomedical Research, Teijin Limited
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Fujii Tadashi
Department Of Botany Faculty Of Science Tokyo University Of Education
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Kondo Noriaki
Laboratory Of Biochemistry Teijin Institute For Biomedical Research Teijin Limited:(present)division
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Watanabe Tsuneo
Laboratory Of Biochemistry Teijin Institute For Biomedical Research Teijin Limited
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