Effects of CO_2 concentration during growth on subsequent photosynthetic CO_2 fixation in Chlorella

概要

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The maximum rate of photosynthetic ^<14>CO_2 fixation (V_<max>) as well as the concentration of CO_2 at which the rate of photosynthetic ^<14>CO_2 fixation attains one-half its maximum velocity (Km) in Chlorella vulgaris 11 h cells was strongly dependent on the concentration of CO_2 continuously provided during the algal growth. The V_<max> (μmoles ^<14>CO_2 fixed/ml pcv・min) and Km (% CO_2) of the algal cells which had been grown in air containing 4% CO_2 (by volume) were ca. 10 and 0.15-0.17, while those in the cells which had been grown in ordinary air (containing 0.04% CO_2) were 7 and 0.05-0.06, respectively. When the concentration of CO_2 in the bubbling gas was lowered from 4 to 0.04% during the algal growth, their photosynthetic kinetics attained the respective lower steady levels after 5-10 hr. On the other hand, when the photosynthetic kinetics were determined 24 hr after raising the concentration of CO_2 from 0.04 to 4%, the V_<max>-and K_m-values were found to have already attained the respective higher levels.
日本植物生理学会の論文