Electron transfer and energy dependent reactions in glutaraldehyde-fixed chloroplasts
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概要
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The effects of GA fixation on electron transfers in photosystems I and II in photosynthesis and energy dependent reactions of chloroplasts, such as changes in light scattering, H^+ uptake and 515-nm absorbance, were investigated. Fixation of chloroplasts with GA resulted in a lowering of the DCIP and MV photoreductions. DCIP photoreduction activity in fixed chloroplasts was not restored in the presence of DPC, an electron donor to photosystem II, but was significantly stimulated by DPC when chloroplasts were fixed after aging. The results suggest that the inhibitory effect of GA fixation on photosystem II differs in its mechanism from those of treatments such as heating, Tris-washing and aging. The oxidation-reduction reaction of P700 was depressed by GA fixation. Energy dependent reactions in fixed chloroplasts were more markedly depressed than were electron transfers. Fixed chloroplasts showed a slight conformational response in the presence of PMS. Analysis of the emission spectrum and the induction of chlorophyll a fluorescence in fixed chloroplasts suggested that the two pigment systems were partially disordered and that the corresponding primary photochemical processes were inhibited.
- 日本植物生理学会の論文
著者
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Oku Tatsuo
Institute Of Biophysics Faculty Of Agriculture Kyushu University
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Tomita Giiti
Institute Of Biophysics Faculty Of Agriculture Kyushu University
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Sugahara Kiyoshi
Division Of Enviromental Biology The National Institute For Enviromental Studies
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Sugahara Kiyoshi
Department Of Biology Faculty Of Science Kyushu University
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