高速液体クロマトグラフィーによる生体試料中のアドリアマイシンとその代謝物の定量(第2報)抽出法による組織の分析

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We developed a favourable method for the determination of adriamycin (ADR) and its metabolites in tissues by use of high porformance liquid chromatography with fluorescence detector. Tissue was homogenized and extracted with a mixture of butanol and toluene. The extract was evaporated in vacuo, and the residue was dissolved with a mixture of phosphate buffer and methanol. An aliquot of this solution was injected onto reversed-phase ODS column, and eluted by a mixture of 1 N formic acid and methanol. The fluorescence signal was monitored at Ex 470 nm and Em 585 nm. Six kinds of tissues from ADR-administered rabbits were analysed by the present method and the total fluorescence method. ADR, adriamycinol and two unidentified metabolites, were detected on chromatograms. Compared with the total fluorescence method, this method is possible to determine each concentration of ADR and its metabolites in tissues. Metabolites are better separated on chromatograms by use of this reversedphase column than normal phase columns previously described.
公益社団法人日本薬学会の論文
1984-06-25