生体試料中の5-Fluorouracilとその代謝物の高速液体クロマトグラフィーによる定量法

概要

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A high performance liquid chromatographic (HPLC) method has been developed to determine simultaneously 5-fluorouracil (5-FU) and its metabolites, 5-fluorodeoxyuridine (FUdR) and 5-fluorouridine (FUR), in various biological samples. 5-FU and its metabolites in these samples were cleaned up by solvent extraction and/or preparative column chromatography. The pretreated samples were dissolved in a mixture of mobile phase for HPLC (see below) and n-hexane (3 : 2). An aliquot of these solutions was injected onto silica gel column (4.6×50 mm, 3 μm, 60 A), and eluted with a mobile phase of ethylacetate-n-hexane-formic acid (88%)-water (60 : 40 : 0.5 : 0.2), and the eluate was monitored at 264 nm. The quantitation limits of 5-FU, FUdR, and FUR were 0.02,0.05,and 0.2μg/ml (or g), respectively. The present method was applied to various samples from 5-FU-administered rabbits and a patient. The results were compared with those determined by a bioassay method.
公益社団法人日本薬学会の論文
1985-11-25