サイトカインを指標とした接触皮膚炎の解析(第1報)

概要

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The study of the participation of T cells and the kinetic studies of cytokines by the RT-PCR method in the challenge phase of contact hypersensitivity reaction (CHR) were performed using skin tissues collected at regular intervals from mice sensitized with 2,4-dinitro-1-fluorobenzene (DNFB). The results obtained from these studies and the further analyses using anti-CD4 and CD8 antibodies showed that the revelation of phenotype of lymph node cells at the reaction site and T lymphocytes at the CHR site were found to play important roles. Furthermore, we examined cytokines formed at the CHR site on the mRNA level. Consequently, it was found that 24 h after the challenge IFN-γ, IL-6 and IL-1β were expressed and 48 h after the challenge IL-2,IL-4 and TNF-α were expressed in addition to these cytokines. The results of the expression of IL-12 and those of the examination using IFN-γ knock out mice suggested that the expression of IFN-γ among several Th1 types of cytokine is especially important for the formation of CHR.
公益社団法人日本薬学会の論文
1997-03-25