オキサゾラムの比色定量について

概要

論文の詳細を見る
A method is described for the colorimetric determination of oxazolam, 10-chloro-2,3,5,6,7,11b-hexahydro-2-methyl-11b-phenylbenzo [6,7]-1,4-diazepino [5,4-b] oxazol-6-one, in pharmaceutical preparations, aqueous solution, urine, and blood serum. Oxazolam was hydrolysed with hydrochloric acid to 2-amino-5-chlorobenzophenone which was colored by the Bratton-Marshall method and the diazo dye was extracted with n-butanol. Color of the azo pigment was stable and absorbance of the solution was measured at 545 mμ. This colorimetric determination was suitable for routine use and has a detectability of 0.5-10μg/ml, it has a very acceptable recovery and reproducibility of the order of 98.5% from aqueous solution, 94.7% from urine, and 100.8% from blood serum. From these results, it was considered that this method can be applied as a reliable determination of oxazolam in forensic chemistry.
公益社団法人日本薬学会の論文
1971-10-30